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Self-Contained Biological Indicators (SCBI) &
Mini Self-Contained Biological Indicators (MSBCI)


Excelsior offers Self-Contained Biological Indicators (SCBI) and Mini Self-Contained Biological Indicators (MSCBI) for monitoring steam sterilisation processes in spore population levels of 105 and 106 and for Ethylene Oxide (EO) and Hydrogen Peroxide processes in a 106 population.Each SCBI & MSCBI unit consists of a plastic vial with a cap, a crushable glass ampoule with recovery media, and a disc inoculated with spores.

SCBIs & MSCBIs are ideal for use in monitoring sterilisation processes in place of traditional Biological Indicator strips, however there is no need for aseptic transfer of the BI to culture media. SCBIs and MSCBIs also offer a shortened incubation period. Traditionally this has been 24 hours, already reduced from 7 days, however Excelsior Scientific is now proud to announce that our SCBIs now offer a rapid readout.

The recovery medium consists of a modified Soybean Casein Digest Broth with pH indicator. Activate for incubation by depressing the cap completely for SCBIs, or squeezing the sides of the MSCBIs and crushing the ampoule. Growth is evident by either turbidity and/ or a colour shift of the media. SCBIs and MSCBIs are labelled with the shorter shelf life of the two components; the inoculated disc and ampoule which have independent expiration periods. Store under room temperature (15˚C - 30˚C) conditions. SCBIs which are 9mm x 48mm are offered in boxes of 50 units. MSCBIs which are 8.3mm x 45.8mm are offered in boxes of 100 units. Each box is accompanied by a Certificate of Analysis and Instructions for Use.

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Self-Contained Biological Indicators


Code Sterilisation Process Organism Pop. Min. Incubation Period
SCS-05E Steam Geobacillus stearothermophilus 105 24 hours
SCS-06E Steam Geobacillus stearothermophilus 106 24 hours
SCE-06E EO Bacillus atrophaeus 106 48 hours


Mini Self-Contained Biological Indicators


Code Sterilisation Process Organism Pop. Min. Incubation Period
MSCS-05E Steam Geobacillus stearothermophilus 105 24 hours
MSCS-06E Steam Geobacillus stearothermophilus 106 24 hours
MSCS-06E EO Bacillus atrophaeus 106 48 hours
MSCS-06E Hydrogen Peroxide Geobacillus stearothermophilus 106 24 hours